Agilent miRNA Expression Profiling Services
Asuragen Services’ addition of the Agilent Human miRNA Array provides our clients with a highly sensitive platform for miRNA analysis. The Agilent platform provides miRNA expression profiles using a robust and highly sensitive microarray. Learn more about how we qualify our methods and staff
Description of Services
Deliverables
Quality Control
Turn-around Time
Additional Services Available
Available miRNA arrays
Catalog Numbers for Agilent miRNA Services
Description of Services
Workflow for Agilent array services:
- Incoming total RNA QC (click here for sample prep services):
- Quantitation and estimation of RNA purity using NanoDrop spectrophotometer readings at 260 and 280 nm
- Assessment of RNA integrity using Agilent 2100 Bioanalyzer to measure RNA integrity number (RIN)
- See our Sample Submission Form and Shipping Instructions for recommended incoming sample QC values
- Target preparation
- Array hybridization, washing, and scanning followed by array QC analysis
- Data summarization (click here for additional analysis services)
- miRInform Data Delivery System (if applicable)
- All raw data files derived from the Agilent Feature Extraction Software
- Analysis Report Tables (in HTML format and MS Excel format, unless table dimensions exceed MS Excel capabilities, in which case CSV files will be provided). These tables include:
- Experimental design
- Raw Signal and Detection Calls
- Statistics (not included if experimental design does not permit statistical analysis, or in data-only projects)
- Pearson Correlation coefficients
- VSN Normalized Array Data
- Comprehensive Annotation
- Optimized for miRNA analysis
- Fast, cost-effective analysis for a wide range of experimental designs
- Accessible DVD format
- High quality figures linked directly to the Sanger mirBase
- Easy to sort tables
Deliverables
As part of our Agilent Human miRNA Array service, you will receive:
For statistical analysis and graphical representation of your entire experiment, Asuragen’s miRInform Data Delivery System is an optional Service that will contain your data in an easy-to-understand package with these benefits:
To ensure security, all information is provided in an electronic form on a DVD. Encryption is available on request. Data may also be delivered by FTP or e-mail.
Quality Control
Agilent does not support the use of in-process controls at the present time. Consequently only incoming sample QC is available.
Turn-around Time
Most projects are completed in two weeks or less. If you have a critical time requirement, we can often meet your need for no additional charge. Read more about our commitment to prompt turn-around times.
Additional Services Available from Asuragen
Available miRNA Arrays
Catalog Numbers for Agilent miRNA Services
| CA0032 | QC, NanoDrop and TaqMan, Total RNA, for miRNA Applications |
| CA0053 | Target Preparation, miRNA, Agilent Human miRNA microarray |
| CA0056 | Array Processing and Scanning, Agilent Human miRNA microarray |
| CA0270 | Agilent Human miRNA microarray |
| CS0020 | miRInform™ Data Delivery System for DiscovArray or Agilent miRNA Expression Profiling |