Low Mass Input Service
Conserve Precious Material: Global Gene Expression Profiling from Limiting Samples
Many expression studies are limited by the samples themselves. Experiments involving clinical samples and bio-fluids are often restricted by cell number, RNA yield, or both. Some tissues or cultures from specialized cells make it difficult to obtain sufficient RNA for sample analysis.
Asuragen has optimized its procedures to enable gene expression profiling using minimal sample input allowing you platform flexibility. Analyze your valuable samples on the Affymetrix or Illumina platform from as little as 100 ng of total RNA.
Regardless of Platform, Asuragen Offers Reliable, Consistent Results
- Standard operating procedures to meet GLP requirements
- All reagents must pass strict quality control standards throughout process
- Extensive operator training and qualification
- Data analysis packages available to accommodate your research needs
Single-round Amplification Using 20-fold Less RNA on the Affymetrix® GeneChip®
Affymetrix’s standard service requires 2 μg of total RNA. Using an enhanced protocol to maximize signal intensity and sensitivity, Asuragen can perform whole genome expression with 100 ng total RNA or less.
Advanced Technologies
- Single-round amplification eliminates bias of 2-round protocols
- Enhanced protocol to maximize signal intensity and sensitivity
- Reproducible results and excellent data quality
Maximize Experimental Replication with the Illumina® Sentrix® BeadChip
Global gene expression profiling typically requires 2 μg or more total RNA, making it difficult to analyze samples of limited mass. Illumina’s Sentrix BeadChip enables expression profiling from low input samples. Asuragen offers a robust and cost-effective service to generate high quality data from this new platform.
- High-performance platform and well-controlled laboratory procedures ensure quality data
- Detection level down to 0.25 pM
- Comprehensive probe panel of > 48,000 gene targets covering RefSeq release 4 (build 34) and alternative splice regions
- Cost effective solution allows you to build better replication into your experiments