AB TaqMan® Gene Expression Services
Using the gold standard AB TaqMan* technology, Asuragen offers the most complete
qRT-PCR service available. Our validated procedures and optimized processes give
you the quality to accurately quantify mRNA expression.
Learn more about how we qualify our methods and staff.
Description of Services
Workflow for all qRT-PCR services:
- Incoming total RNA QC (click here for
sample prep services):
- Quantitation and estimation of RNA purity using NanoDrop spectrophotometer readings at 260 and 280 nm
- qRT-PCR assessment of housekeeping genes as a surrogate measure of
overall mRNA abundance
- See our
Sample Submission Form and
Shipping Instructions for recommended incoming sample QC values
- Reverse-transcription by random priming (default), oligo-dT priming,
or gene specific priming
- Real-time detection via TaqMan primer & probe technology in triplicate
Workflow for all qPCR services:
- Incoming DNA QC by
NanoDrop spectrophotometry
- Real-time detection via TaqMan primer & probe technology in triplicate
Deliverables
As part of our TaqMan Gene Expression services, you will receive a complete data
and experimental report containing:
mRNA — Relative Abundance
- Raw CT values
- ΔCT (normalization of each target gene to an endogenous
control gene(s))
- ΔΔCT (calibration of normalized values to control sample)
- Excel spreadsheet
TaqMan Arrays
- Raw CT values
- ΔCT (normalization of each target gene to an endogenous
control gene(s))
- ΔΔCT (calibration of normalized values to control sample)
- Excel spreadsheet
To ensure security, all information is provided in an electronic form on a DVD.
Encryption is available on request. Data may also be delivered by FTP or e-mail.
Quality Control
During our processes, we monitor many QC checkpoints as recommended by the Applied
Biosystems’ procedures. As much as possible, we utilize spike-in controls, in-process
sample controls, and process or reagent batch controls. Should any sample-specific
QC measure result in an “alert” or “fail” reading, we will contact you to discuss
available options. These rigorous procedures enable Asuragen to be the only service
provider to offer a Data Quality Guarantee:
- If, for any reason, a qualified sample does not pass our process
control specifications, we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Data Analysis
Asuragen is very experienced with qRT-PCR-based expression analysis. As part of
the TaqMan services, we summarize the data and provide you with a complete data
and experimental report. Relative abundance is standard for TaqMan Gene Expression
Assays. Absolute quantitation is available on a custom basis.
Turn-around Time
Most projects are completed in one to two weeks. If you have a critical time
requirement, we can often meet your need for no additional charge. Read more about our commitment
to prompt turn-around times.
Additional Services Available
Formats:
Individual assays or arrays (384-well microfluidic cards with pre-loaded assays
and sample parts for minimal error from pipetting)
TaqMan arrays: an Ideal Screening Technology
TaqMan Arrays are ideal for screening biomarkers and toxicology
panels and for analyzing pathways, target classes, and complete disease sets. Taqman
arrays cards are pre-loaded for reliable performance and reproducible results.
Choose from:
TaqMan® Gene Signature Arrays,
TaqMan® Gene Sets or a
TaqMan® Custom Arrays.
Product Bulletin:
TaqMan® Custom Arrays
More TaqMan® Array product literature
Available TaqMan Products:
mRNA
TaqMan Gene Expression Assays and Arrays Choose from over
1,000,000 pre-designed probe/primer sets.
TaqMan Gene Expression Assays
Species
|
Number of Assays
|
|
H. sapiens
|
197,614
|
|
M. musculus
|
175,509
|
|
R. norvegicus
|
150,275
|
|
C. familiaris (Dog)
|
54,639
|
|
M. mulatta (Rhesus Macaque)
|
14,837
|
|
A. thaliana
|
97,879
|
|
B. taurus (Cow)
|
65,408
|
|
G. gallus (Chicken)
|
47,613
|
|
O. cuniculus (Rabbit)
|
3,428
|
|
S. scrofa (Pig)
|
6,866
|
|
C. elegans
|
92,687
|
|
D. melanogaster
|
41,607
|
|
D. rerio (Zebrafish)
|
63,694
|
|
|
|
|
Total Number of Assays*
|
1,012,056
|
TaqMan®
Gene Expression Assays Product Guide (PDF, 1.0 MB)
Assay Development and Validation
Asuragen offers custom TaqMan mRNA assay design with proven success on difficult
samples such as degraded RNA from FFPE. Validation of off-the-shelf or custom assays
to measure absolute performance is also available as a custom service.
Catalog Numbers for Gene Expression qRT-PCR Services
|
CA0030
|
AB TaqMan Service for mRNA, individual assays, RT-PCR
|
|
CA0035
|
AB TaqMan Assay Validation, mRNA
|
|
CA0046
|
AB TaqMan array for mRNA, PCR only
|
|
CA0047
|
RT for TaqMan mRNA array, random-primed
|
|
CA0037
|
QC, NanoDrop and TaqMan, Total RNA, for mRNA PCR
|
|
CS0016
|
Fee, TaqMan mRNA qRT-PCR Probe & Primer Design
|
*This test is performed pursuant to license arrangements with Roche Molecular Systems,
Inc. and Applera Corporation.