Applications and Protocols
Armored Technology™: Applications and Protocols
The main advantage of Armored RNA controls is that the packaged RNA is stable and
ribonuclease-resistant in plasma and other matrices. The RNA is compatible with
any RNA-based clinical assay after sample extraction.
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Applications
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Armored RNA®
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Armored RNA®
Quant™
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Assay Development
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Laboratories lack access to all viruses
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X
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X
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Daily Controls
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Positive controls to verify assay performance
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X
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X
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Internal Controls
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Normalize data
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X
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X
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Calibrators
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Determine mean range of assay
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X
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Quantitate
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Quantitate against standard curve
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X
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Extraction Control
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Determine extraction efficiency
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X
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X
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Direct Detection of Target
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External RT-PCR Control
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- Heat lyse Armored RNA or Armored RNA Quant at 75°C for 3 minutes
- Place on ice
- Detect by RT-PCR
- Use 5 µL of heat-lysed Armored RNA or desired amount of Armored RNA Quant for a
20 µL RT reaction
- Use 2 to 5 µL of RT for a 25 to 50 µL PCR reaction
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Sample Quantitation – Standard Curve
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- Spike a known quantity of Armored RNA Quant into negative matrix
- Prepare standard curve by 10-fold serial dilutions (at least 5 points)
- Extract standard curve along with patient samples via kit of choice
- Perform 3-5 replicates
- Detect by qRT-PCR
- Quantitate samples against standard curve
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Specimen Extraction Efficiency
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Internal Control (IC)
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Sequence of IC different from that of target
- Spike a known quantity of Armored RNA or Armored RNA Quant into patient specimen
- Extract sample via commercial kit of choice
- Detect IC by RT-PCR
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External Extraction Control
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- Spike either Armored RNA or Armored RNA Quant into negative matrix (e.g. serum,
plasma)
- Extract sample via kit of choice
- Detect target by RT-PCR
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